Bulg. J. Phys. vol.27 no.S3 (2000), pp. 203-206

Microwave Impact on Enzyme Lactate Dehydrogenase

A. Hategan1, D. Martin1, A. Popescu1, C. Oproiu1, I. Margaritescu2
1National Institute for Lasers, Plasma and Radiation Physics (NILPRP), Electron Accelerators Laboratory, 111 Atomistilor St., P.O.Box: MG36, 76900 Bucharest, Romania
2"Carol Davila" University of Medicine and Pharmacy, Bucharest , Romania
Abstract. This study provides experimental data concerning the effects of microwave irradiation on enzyme lactate dehydrogenase. Crystallized lactate dehydrogenase in ammonium sulphate solution was irradiated in ice-water bath at 0℃ with 2.45 GHz microwaves at 310 W, 400 W, and 550 W power levels, in the time range (0-40 s), the final temperature of the samples being determined in order to interpret the results. Our results showed a uniform decrease of the enzymatic activity accompanying the increase of the temperature for the 310 W irradiated samples. The 400 W, and 550 W irradiated samples exhibited a non-linear behaviour as the final temperature rises, with no correlation between the temperature rise in the sample by microwave heating and the enzyme activity, suggesting the major contribution of the nonthermal effects of microwave irradiation.

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